Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.abb.2010.06.012
Title: Instability of, and generation of hydrogen peroxide by, phenolic compounds in cell culture media
Authors: Long, L.H.
Hoi, A. 
Halliwell, B. 
Keywords: Apigenin
Cell culture
Curcumin
DMEM
Hesperetin
Hydrogen peroxide
Naringenin
Resveratrol
Tyrosol
Issue Date: 2010
Source: Long, L.H., Hoi, A., Halliwell, B. (2010). Instability of, and generation of hydrogen peroxide by, phenolic compounds in cell culture media. Archives of Biochemistry and Biophysics 501 (1) : 162-169. ScholarBank@NUS Repository. https://doi.org/10.1016/j.abb.2010.06.012
Abstract: Many papers in the literature have described complex effects of flavonoids and other polyphenols on cells in culture. In this paper we show that hydroxytyrosol, delphinidin chloride and rosmarinic acid are unstable in three commonly-used cell culture media (Dulbecco's modified Eagle's medium (DMEM), RPMI 1640 (RPMI) and Minimal Essential Medium Eagle (MEM)) and undergo rapid oxidation to generate H2O2. This may have confounded some previous studies on the cellular effects of these compounds. By contrast, apigenin, curcumin, hesperetin, naringenin, resveratrol and tyrosol did not generate significant H2O2 levels in these media. Nevertheless, curcumin and, to a lesser extent, resveratrol (but not tyrosol) were also unstable in DMEM, so the absence of detectable H2O2 production by a compound in cell culture media should not be equated to stability of that compound. Compound instability and generation of H2O2 must be taken into account in interpreting effects of phenolic compounds on cells in culture. © 2010 Elsevier Inc. All rights reserved.
Source Title: Archives of Biochemistry and Biophysics
URI: http://scholarbank.nus.edu.sg/handle/10635/28604
ISSN: 00039861
10960384
DOI: 10.1016/j.abb.2010.06.012
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