Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.neulet.2005.12.011
Title: Early induction of calpains in rotenone-mediated neuronal apoptosis
Authors: Chen, M.J.
Yap, Y.W. 
Choy, M.S. 
Koh, C.H.V. 
Seet, S.J.
Duan, W. 
Whiteman, M. 
Cheung, N.S. 
Keywords: Apoptosis
Calpains
Caspases
Cell cycle re-entry
Parkinson's disease
Rotenone
Issue Date: 2006
Source: Chen, M.J., Yap, Y.W., Choy, M.S., Koh, C.H.V., Seet, S.J., Duan, W., Whiteman, M., Cheung, N.S. (2006). Early induction of calpains in rotenone-mediated neuronal apoptosis. Neuroscience Letters 397 (1-2) : 69-73. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neulet.2005.12.011
Abstract: Rotenone is an inhibitor of mitochondrial complex I that produces a model of Parkinson's disease (PD), where neurons undergo apoptosis by caspase-dependent and/or caspase-independent pathways. Inhibition of calpains has recently been shown to attenuate neuronal apoptosis. This study aims to establish for the first time, the time-point of calpain activation with respect to the caspase activation and the possibility of cell cycle re-entry in rotenone-mediated cell death. Immunoblot results revealed calpain activation occurred at 5, 10 h prior to caspase-3 activation (at 15 h), suggesting calpain activation was an earlier cellular event compared to caspase activation in the rotenone-mediated apoptosis. In addition, an upregulation of phospho-p53 was observed at 21 h. However, no expression or upregulation of cell cycle regulatory proteins including cdc25a, cyclin-D1 and cyclin-D3 were observed, strongly suggesting that cell cycle re-entry did not occur. These findings provide new insights into the differential patterns of calpain and caspase activation that result from rotenone poisoning and which may be relevant to the therapeutic management of PD. © 2005 Elsevier Ireland Ltd. All rights reserved.
Source Title: Neuroscience Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/28564
ISSN: 03043940
DOI: 10.1016/j.neulet.2005.12.011
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

32
checked on Dec 14, 2017

WEB OF SCIENCETM
Citations

29
checked on Nov 16, 2017

Page view(s)

210
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.