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|Title:||Different cytotoxic and clastogenic effects of epigallocatechin gallate in various cell-culture media due to variable rates of its oxidation in the culture medium|
|Keywords:||Cell culture media|
|Citation:||Long, L.H., Halliwell, B., Kirkland, D., Whitwell, J. (2007). Different cytotoxic and clastogenic effects of epigallocatechin gallate in various cell-culture media due to variable rates of its oxidation in the culture medium. Mutation Research - Genetic Toxicology and Environmental Mutagenesis 634 (1-2) : 177-183. ScholarBank@NUS Repository. https://doi.org/10.1016/j.mrgentox.2007.07.009|
|Abstract:||Positive genotoxicity results are often observed using mammalian cells in culture with agents that are not in vivo genotoxins. We here illustrate one possible explanation: interaction of test chemicals with the cell-culture media used. We find that the toxicity and clastogenicity of epigallocatechin gallate (EGCG) to Chinese Hamster ovary (CHO) cells is affected by the culture medium used and appears largely or entirely due to variable rates of formation of hydrogen peroxide (H2O2) by chemical reactions of EGCG with the culture media. Catalase decreased EGCG toxicity substantially. Of seven different types of commonly used media evaluated, F-10 and F-12 nutrient mixtures were the least prone to produce this artefact. Although it generated H2O2 in the culture media, ascorbate was not toxic to CHO cells because the H2O2 levels achieved were insufficient to kill these cells. Thus, the culture medium, the cell type and the presence or absence of catalase (e.g. its variable amounts in S9 fractions) must be taken into account in in vitro genotoxicity testing. © 2007 Elsevier B.V. All rights reserved.|
|Source Title:||Mutation Research - Genetic Toxicology and Environmental Mutagenesis|
|Appears in Collections:||Staff Publications|
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