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Title: Mutagenesis studies of the Pseudomonas global regulator, MorA, and cloning of its signaling pathway member, Adenylate Cyclase
Keywords: MorA;flagellar development;biofilm formation;Adenylate cyclase;cloning;mutagenesis;
Issue Date: 22-Jan-2008
Citation: THATTENGAT JYOTHILAKSHMI MENON (2008-01-22). Mutagenesis studies of the Pseudomonas global regulator, MorA, and cloning of its signaling pathway member, Adenylate Cyclase. ScholarBank@NUS Repository.
Abstract: Bacteria can exist in free living state (planktonic state) or as part of surface associated multicellular communities called biofilms. Their ability to shift between these two states is determined by various environmental factors including nutrient levels, moisture etc. Regulation of flagella formation is critical for both swimming in liquid and viscous media, swarming along surfaces as well as biofilm formation. Many factors play a role in this regulation including local and global regulators and quorum sensing. MorA is one such global regulator of flagellar development in Pseudomonas, whose mutation results in derepression of flagellar development that leads to enhancement of motility and chemotaxis. Our laboratory is working extensively at uncovering the various aspects of MorA mediated signaling pathways. In this aspect, previous workers had screened a set of hypermotile morA revertant mutants and identified a few genes downstream of morA. One of these genes, an Adenylate cyclase (PpAC) has been cloned and sequenced fully in this study. Possible roles in the morA signaling pathway are discussed. In addition, some site directed mutants of morA were created and protein expression studies were carried out. A comprehensive account of flagellar biosynthesis, motility, and role of various regulators (including MorA) is also discussed here.
Appears in Collections:Master's Theses (Open)

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