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Title: Functional study of zebrafish Udu and its relationship to the Notch signaling pathway
Keywords: zebrafish, p53, apoptosis, replication, DNA damage, cell cycle
Issue Date: 29-Apr-2009
Source: LIM CHIAW HWEE (2009-04-29). Functional study of zebrafish Udu and its relationship to the Notch signaling pathway. ScholarBank@NUS Repository.
Abstract: We used a zebrafish mutant; ugly duckling (udutu24) isolated in the 1996 Tubingen genetic screen and showed that its extensive p53-dependent apoptosis involved the Atm-Chk2 pathway. Our FACS analysis showed that the loss of udu function resulted in defective cell cycle progression and comet assay indicated the presence of increased DNA damage in udutu24 mutants. Yeast two-hybrid and co-immunoprecipitation data demonstrated that PAH-L (Paired Amphipathic N1-Helix like) repeats and SANT-L (SW13, ADA2, N-Cor and TFIIIB like) domain of Udu interacts with MCM3 and MCM4. Furthermore, Udu is co-localized with BrdU and heterochromatin during DNA replication, suggesting a role in maintaining genome integrity.Evidence from our studies also showed that the udu may play a role in regulating Notch signaling pathway.
Appears in Collections:Ph.D Theses (Open)

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