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Title: Crystal structure analysis of PilS, a type lVb pilin from Salmonella typhi
Keywords: Type IV pilin, Salmonella typhi, CFTR, crystal structure
Issue Date: 19-Dec-2007
Citation: MANIKKOTH BALAKRISHNA ASHA (2007-12-19). Crystal structure analysis of PilS, a type lVb pilin from Salmonella typhi. ScholarBank@NUS Repository.
Abstract: Type IV pili are found on many bacterial species and play a crucial role in pathogenesis, as they are required for the attachment of bacteria to target host cells. The Type IV pilus of the entero-pathogenic bacteria Salmonella typhi is believed to be a major adhesion factor during the entry of this pathogen into the gastrointestinal epithelial cells. Understanding the subunit structure and assembly architecture that produce these extraordinary filaments is crucial for understanding pilus functions and for designing vaccines and therapeutics that can be directed to blocking pilus activities. The target receptor for the S. typhi pilus is a stretch of 10 residues from the first extra-cellular domain of CFTR. In the present study, the structure of N PilS and the N PilS/CFTR peptide complex have been solved by the MAD and molecular replacement methods, respectively. The complex structure gives us insight into the potential residues that are essential for receptor binding. Furthermore, we have also explored the role of a conserved disulfide bridge in pilus formation.
Appears in Collections:Ph.D Theses (Open)

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