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Title: Mitochondrial proteomics of colorectal cancer cells: study of the effects of butyrate and subcellular expression of mortalin
Keywords: mitochondria, proteomics, cancer, butyrate, subcellular, mortalin
Issue Date: 15-Jun-2008
Citation: KUAH LI FANG (2008-06-15). Mitochondrial proteomics of colorectal cancer cells: study of the effects of butyrate and subcellular expression of mortalin. ScholarBank@NUS Repository.
Abstract: Colorectal cancer is the most prevalent cancer in developed countries, accounting for over half a million of deaths annually. Butyrate, a fermentation product of dietary fibre, is a candidate chemotherapeutic agent which specifically induces growth arrest, differentiation and apoptosis of cancer cells in vitro and in vivo. The mitochondria have been shown to play a pivotal role in these butyrate-induced effects, but the precise mechanisms remain unclear. In this study, we investigated mitochondrial proteome changes induced by butyrate in the HCT 116 colon cancer cells, using two-dimensional difference gel electrophoresis. Butyrate altered the expression of 18 well-characterised mitochondrial proteins including metabolic and redox enzymes, respiratory complex subunits, regulators of protein import and translation, and death-inducing factors. One of these proteins, mortalin, was further characterised. Our results showed that a specific mortalin isoform was preferentially down-regulated in the mitochondrial and nuclear fractions of HCT 116, but not in the more resistant HT-29 cell line, upon butyrate-induced apoptosis. This may imply that specific mortalin isoforms are associated with susceptibility to apoptosis.
Appears in Collections:Master's Theses (Open)

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