Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/28269
Title: Mechanical Insights into the Physiological Functions of Claudin Mediated Adhesion at Tight Junctions
Authors: LIM TONG SENG
Keywords: claudin, tight junctions, cell-cell adhesion, molecular force spectroscopy, atomic force microscopy, biophysics
Issue Date: 18-Dec-2008
Source: LIM TONG SENG (2008-12-18). Mechanical Insights into the Physiological Functions of Claudin Mediated Adhesion at Tight Junctions. ScholarBank@NUS Repository.
Abstract: Intercellular adhesion molecules play an important role in regulating several cellular processes such as proliferation, differentiation and inflammation. The role of selective paracellular permeability to epithelial monolayer is contributed by a family of transmembrane proteins called claudins (Cldns) at TJs. Their ability to regulate the paracellular pathway is determined by the extracellular loops of Cldns and highly influenced by changes in extracellular pH. Using single molecular force spectroscopy, Cldn1 was found to dissociate at a much faster rate than E-cadherins. The first extracellular loop of Cldn2 (C2E1) was shown to be the major molecular determinants of trans-interactions involving Cldn2. Comparison of adhesion kinetics under different pH conditions further revealed that Cldn2/Cldn2 is relatively more stable in neutral environment. Our results provide mechanical insights into the molecular determinants as well as environmental factors that regulate Cldn-mediated adhesion at tight junctions.
URI: http://scholarbank.nus.edu.sg/handle/10635/28269
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
cover.pdf9.93 kBAdobe PDF

OPEN

NoneView/Download
01Chap.pdf45.12 kBAdobe PDF

OPEN

NoneView/Download
02Chap.pdf1.14 MBAdobe PDF

OPEN

NoneView/Download
Appendix_A.pdf259.3 kBAdobe PDF

OPEN

NoneView/Download
Appendix_B.pdf1.29 MBAdobe PDF

OPEN

NoneView/Download
Appendix_C.pdf1.05 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

159
checked on Dec 11, 2017

Download(s)

754
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.