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Title: Protein and Cell Micropatterning and its Integration With Micro/Nanoparticles Assembly
Keywords: Protein & Cell Micropatterning; Colloidal Assembly; Microfabrication
Issue Date: 31-Mar-2008
Citation: YAP FUNG LING (2008-03-31). Protein and Cell Micropatterning and its Integration With Micro/Nanoparticles Assembly. ScholarBank@NUS Repository.
Abstract: Protein and cell micropatterning have important applications in the development of biosensors and lab-on-a-chip devices, microarrays, tissue engineering and fundamental cell biology studies. The conventional micropatterning techniques involve patterning over a planar substrate. In this thesis, the introduction of topographical features on the adhesive regions to enhance proteins and cells behavior is proposed. A textured substrate for proteins and cell adhesion is created by assembly of micro and nanoparticles into an array of microwells on a silicon substrate. The topography can be controlled by varying the size and density of the particles. Firstly, a technique of generating spatial arrangement of particles on a non-fouling background is developed. This is achieved by using a bi-functional template which can overcome the conflict between the pre-requisites for particles assembly and micropatterning of biomolecules. A fluidic chamber was designed to control the movement of the particle suspension across the template so as to attain uniform particles array over large area.After assembly of particle, proteins can be conjugated to the curve surface of the particles. Attachment of biomolecules on particles surfaces can increase the density of biomolecules and proteins can retain its native structure and function better than on a planar surface. Alternatively, cell micropatterning can be performed and it was shown that the textured surface helped to improve the proliferation and adhesion and of cells.
Appears in Collections:Ph.D Theses (Open)

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