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Title: Transcriptomic pathways of apoptosis induced by denn gene downregulation in CNE-2 Nasopharyngeal carcinoma cells
Keywords: Transcriptomic pathways, apoptosis, anti-sense phosphorotioate, Differentially expressed in normal and neoplastics cells (DENN), nasopharyngeal carcin
Issue Date: 18-Mar-2008
Source: CHAI SING HUI, ANTHEA (2008-03-18). Transcriptomic pathways of apoptosis induced by denn gene downregulation in CNE-2 Nasopharyngeal carcinoma cells. ScholarBank@NUS Repository.
Abstract: Nasopharyngeal carcinoma (NPC) is a malignant tumour arising from the nasopharyngeal epithelium. The current treatment of radiotherapy and chemotherapy are effective but results in many severe adverse effects affecting the quality of life of patients. In search of more specific medication for NPC, novel approaches for the treatment have been extensively investigated. The present work employed gene therapy method by downregulating DENN (Differentially expressed in normal and neoplastic cells) which has been found to have role pro-survival function in CNE-2 cells. Using anti-sense technology, we found blocking of DENN expression induced apoptosis. Our microarray, real-time PCR and western blot data showed the involvement of DENN in TNF mediated signaling leading to apoptosis. Besides, involvement of novel signaling pathway Rab3a and Rab5 were identified and disturbed. The dysregulation of many genes present in mitochondria indicated the mitochondrial damage, which was evident through transmission electron microscopy. These studies concluded that targeting DENN may have therapeutic role in the treatment of nasopharyngeal cancer.
Appears in Collections:Master's Theses (Open)

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