Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/28217
Title: Perturbational metabolomics to study interrelationships among metabolic pathways in Arabidopsis
Authors: REUBEN SHEELA
Keywords: Flavonoids, lignin pathway, isoprenoid pathway, ttg1, tt6, transgenic plants
Issue Date: 24-Aug-2008
Source: REUBEN SHEELA (2008-08-24). Perturbational metabolomics to study interrelationships among metabolic pathways in Arabidopsis. ScholarBank@NUS Repository.
Abstract: Knowledge of relationships among metabolic pathways is essential in understanding the complexity of cellular responses. Here, in order to study metabolic pathway relationships, we have perturbed plant metabolic pathways using an oxidoreductase PhpA from Pseudomonas putida, which converts quercetin to naringenin. Transgenic expression of PhpA in Arabidopsis resulted in bidirectional channeling both into downstream anthocyanins pathway and upstream branched lignin pathway which was identified using metabolomics approach. Both short and long distance perturbation effects were observed in phenylpropanoid, isoprenoid and nucleotide pathways. Perturbation led to changes in both substrate and gene expression levels of biosynthetic as well as regulatory genes. Loss-of-function mutants, tt6 and ttg1 lines showed metabolic profile similarity with PhpA transgenic, thus showing that long range effects can be observed in different perturbations. Both short and long range perturbational response of metabolites and multiple regulatory proteins of biosynthetic pathways strongly suggest a higher level of control of these pathways.
URI: http://scholarbank.nus.edu.sg/handle/10635/28217
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