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Title: Planar electrochromotography and electromembrane protein separation using nanoporous alumina materials
Authors: HE LIN
Keywords: electrochromotography electromembrane protein separation nanoporous alumina
Issue Date: 12-Sep-2008
Citation: HE LIN (2008-09-12). Planar electrochromotography and electromembrane protein separation using nanoporous alumina materials. ScholarBank@NUS Repository.
Abstract: By electronic enodization, the pore size and pore intensity of nanoporous alumina was precisely adjusted. Surface treatment with different organic acids was carried out on home-made glass supported alumina surface. X-ray Photoelectron Spectroscopy AXIS Instrument was used to characterize the surface modification. Successful grafting on etched glass-supported alumina surface was achieved. The variation of surface property after chemical treatment was studied.Application of nano-porous alumina has been explored for both off-line and on-line planar electrochromatography protein separation. Protein separation condition was optimized by varying alumina pore size, pore depth, surface properties as well as the pH value of buffer solution. The mechanism of planar electrochromatography protein separation was studied. Multi Nano-channel Electromembrane Separation (MNES) technique with an on-site horizontal set-up was demonstrated. Different factors affecting separation efficiency were studied and MNES protein separation mechanism was proposed and studied. Separation of BSA and LYS across polarized alumina membrane was achieved.
Appears in Collections:Master's Theses (Open)

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