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Title: Functional characterization of HGF and its receptor C-Met in zebrafish development
Keywords: HGF c-met zebrafish development liver somitogenesis
Issue Date: 4-Jun-2008
Citation: SHENG DONGLAI (2008-06-04). Functional characterization of HGF and its receptor C-Met in zebrafish development. ScholarBank@NUS Repository.
Abstract: Hepatocyte Growth Factor (HGF) is a pleiotropic factor that affects many aspects of biological functions through activation of its transmembrane tyrosine kinase receptor c-Met. In this study, we used zebrafish as a vertebrate model to study the developmental roles of hgf and its receptor c-met. In zebrafish, gene duplication in the teleost lineage resulted in two co-orthologs of hgf, named as hgfa and hgfb. Expression analysis by qRT-PCR, whole mount in-situ hybridization and functional analysis by morpholino antisense oligonucleotides knockdown revealed zebrafish hgf genes are expressed in restricted and non-overlapping expression patterns and regulate distinct processes during development. hgfa was shown to be involved in somitogenesis while hgfb is required for liver development. These studies expanded our understanding of HGF/c-Met in vertebrate development and established the foundation for further studies of their molecular mechanisms of action in various organs as well as their regulation of expression.
Appears in Collections:Ph.D Theses (Open)

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