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Title: Biocompatible polyelectrolyte fibers for viral gene delivery
Keywords: Non-covalent assembly, interfacial, DNA, amphipathic peptides, polyelectrolyte fibers, baculovirus, sustained release.
Issue Date: 26-May-2008
Citation: HARSH JOSHI (2008-05-26). Biocompatible polyelectrolyte fibers for viral gene delivery. ScholarBank@NUS Repository.
Abstract: The phenomenon of self-assembly of biomolecules has opened new channels for interdisciplinary research. These self-assembled systems find a wide range of applications like scaffolds for cell culture, tissue regeneration and drug and gene delivery devices. In this study, we have demonstrated the interfacial non-covalent assembly of plasmid DNA and a set of amphipathic peptides to form polyelectrolyte fibers. These fibers can be used to encapsulate baculovirus, which is a versatile gene delivery vector. We have shown that fibers made from functionalized peptides are suitable for cell adhesion and that these fibers can induce localized gene expression. We have also demonstrated a delayed release of viral particles in serum-free as well as serum containing medium thereby opening the possibility of providing protection to the virus via incorporation in fibers.
Appears in Collections:Master's Theses (Open)

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