Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/28166
Title: Comparison of Different Systems for Chondrogenic Differentiation of Human Embryonic Stem Cells: The Effect of TGF-beta1
Authors: SUI LIN
Keywords: hESC-direct, EB-derived, chondrogenic differentiation, TGF-beta1, micromass culture, pluripotency
Issue Date: 28-Apr-2008
Source: SUI LIN (2008-04-28). Comparison of Different Systems for Chondrogenic Differentiation of Human Embryonic Stem Cells: The Effect of TGF-beta1. ScholarBank@NUS Repository.
Abstract: In this study, we demonstrated the potential of hESCs to undergo chondrogenic differentiation under different culture environment and examined the effect of transforming growth factor beta1 (TGFbeta1). When undifferentiated hESCs were seeded directly as micromass without embryoid body (EB) formation (referred to as ESC-direct), the serum-free basic chondrogenic media alone (referred as control) was sufficient to induce chondrogenesis, indicated by increased expression of chondrogenic markers. With treatment of TGFbeta1, down-regulation of chondrogenic markers was observed. Further investigation demonstrated that TGFbeta1 prolonged the pluripotency markers Oct4 and Nanog during differentiation. In comparison, chondrogenesis in EB-derived micromass was not drastically affected by TGFbeta1. Furthermore, the study also showed that chondrogenesis in EB-derived micromass was exquisitely sensitive to cell seeding density. Extend of chondrogenesis was diminished with increased seeding density. Unlike mesenchymal stem cells, hESCs cultured as pellets did not undergo chondrogenesis with no detection of aggrecan or collagen II.
URI: http://scholarbank.nus.edu.sg/handle/10635/28166
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00-Title page.pdf49.01 kBAdobe PDF

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01-acknowlegement-table of content-summary-list of figure.pdf125.49 kBAdobe PDF

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02-CHAPTER1 Literature Review p1-23.pdf142.14 kBAdobe PDF

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03-CHAPTER2 Material_and_Method p24-33.pdf97.79 kBAdobe PDF

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04-CHAPTER3 results p34-61.pdf1.69 MBAdobe PDF

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05-CHAPTER4&5 dicussion&conclusion p62-75.pdf163.92 kBAdobe PDF

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06-CHAPTER6 appendices p76-77.pdf108.35 kBAdobe PDF

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07-CHAPTER 7 reference p78-90.pdf115.49 kBAdobe PDF

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