Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/28150
Title: Identification of Factors Involved in the Maintenance of Embryonic Stem Cell Self Renewal and Pluripotency
Authors: LIM KEAT SANDY
Keywords: ES cells, Lefty, Nodal signaling, Morphogen, self renewal, pluripotency
Issue Date: 25-Sep-2008
Source: LIM KEAT SANDY (2008-09-25). Identification of Factors Involved in the Maintenance of Embryonic Stem Cell Self Renewal and Pluripotency. ScholarBank@NUS Repository.
Abstract: The factors and mechanisms governing embryonic stem cell self renewal remain poorly understood, thereby limiting biomedical applications. Lefty2, a Nodal antagonist, was first identified as being important for maintaining pluripotency in a candidate gene RNAi screen in mouse ES cells. Using a loss of function approach, targeted depletion of Lefty2 with shRNAs caused dramatic ES cell differentiation towards mesendodermal lineages. Lefty2 was found to be secreted by mouse ES cell as a negative feedback mechanism to autoregulate autocrine Nodal signals. How Nodal signaling influence ES cell fate decisions remains poorly understood and is addressed here. It was found that Nodal signaling is able to arbitrate at least three distinct fate decisions in ES cells. Perturbation of endogenous signaling in ES cells leads to their exit from self renewal towards differentiation. Increasing Nodal signals above basal levels causes ES cell differentiation into meso/mesendodermal lineages while repression of signaling induces trophectodermal differentiation.
URI: http://scholarbank.nus.edu.sg/handle/10635/28150
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