Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/28147
Title: Paired-End Tags For Unravelling Genomic Elements And Chromantin Interactions
Authors: MELISSA JANE FULLWOOD
Keywords: Genomics Chromatin Interactions Sequencing Transcription
Issue Date: 8-May-2009
Source: MELISSA JANE FULLWOOD (2009-05-08). Paired-End Tags For Unravelling Genomic Elements And Chromantin Interactions. ScholarBank@NUS Repository.
Abstract: Comprehensive understanding of functional elements in the human genome will require thorough interrogation and comparison of individual human genomes and genomic structures. In particular, one of the most important questions in gene expression regulation is how remote control of transcription regulation in a complex genome is organized. The Paired-End Tag (PET) strategy involves extraction of paired short tags from the ends of linear DNA fragments for ultra-high-throughput sequencing. In addition to new methods of constructing PETs, here I show a novel application of PET in understanding molecular interactions between distant genomic elements. Using this Chromatin Interaction Analysis with Paired-End Tag (ChIA-PET) sequencing method, I present the first-ever global estrogen receptor N1-mediated human interactome chromatin map. I show that chromatin interactions are important in gene regulation. With its versatile and powerful nature, the PET sequencing strategies and the new application, ChIA-PET, have a bright future ahead.
URI: http://scholarbank.nus.edu.sg/handle/10635/28147
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Chapter 3 Binding sites.xls711.5 kBMicrosoft Excel

OPEN

NoneView/Download
Chapter 3 Genes associated with high confidence interactions.xls2.52 MBMicrosoft Excel

OPEN

NoneView/Download
Chapter 3 Interactions.xls120 kBMicrosoft Excel

OPEN

NoneView/Download
Chapter 4 Binding sites.txt387.5 kBText

OPEN

NoneView/Download
Chapter 4 Genes associated with interaction regions.xls248.5 kBMicrosoft Excel

OPEN

NoneView/Download
Chapter 4 Interaction regions.xls154 kBMicrosoft Excel

OPEN

NoneView/Download
Chapter 4 Interactions.txt176.21 kBText

OPEN

NoneView/Download
Chapter 4 TRANSFAC analysis.xls36.5 kBMicrosoft Excel

OPEN

NoneView/Download
Chapter 6 DNA sequences and notes.xls41.5 kBMicrosoft Excel

OPEN

NoneView/Download
ChIA-PET browser manual.doc2.03 MBMicrosoft Word

OPEN

NoneView/Download
ChIA-PET library construction protocol.doc184.5 kBMicrosoft Word

OPEN

NoneView/Download
Selection-MDA GIS-PET library construction protocol.doc153.5 kBMicrosoft Word

OPEN

NoneView/Download
Statement of work performed by myself for this thesis.doc4.14 MBMicrosoft Word

OPEN

NoneView/Download
Thesis.pdf3.63 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

286
checked on Dec 8, 2017

Download(s)

316
checked on Dec 8, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.