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Title: Investigation of microfluidics in channels and tissues by fluorescence correlation spectroscopy (FCS)
Keywords: fluorescence correlation spectroscopy FCS microfluidic flow
Issue Date: 7-May-2009
Citation: PAN XIAOTAO (2009-05-07). Investigation of microfluidics in channels and tissues by fluorescence correlation spectroscopy (FCS). ScholarBank@NUS Repository.
Abstract: Fluorescence correlation spectroscopy is an optical technique with single molecule sensitivity that measures diffusion, concentration and molecular interactions. It has also been applied to microo, uidic o, ow measurements in microchannels, plant tissues and small animals. The method uses small molecules as a probe to avoid the possible obstruction of microchannels, and it has a higher spatial resolution than all the other well-established techniques. A spatial o, ow proo, le across the dorsal aorta was characterized as a verio, cation of FCS o, ow measurements with high resolution in tissues. With a custom-built o, uorescence correlation microscope system, the directions of microo, uidic o, ows in the isolated liver, liver slice, cell-culture microchannel perfusion system were measured by scanning FCS. Next, blood o, ow measurement in zebrao, sh embryo by scanning FCS was demonstrated as well.
Appears in Collections:Ph.D Theses (Open)

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