Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/28132
Title: Assessment of mitomycin C-induced cytotoxicity and genotoxicity utilizing somatic progenies of human embryonic stem cells
Authors: LU KAI
Keywords: embryonic stem cell, cytotoxicity, genotoxicity, ISO, OECD, mitomycin C
Issue Date: 18-Dec-2007
Source: LU KAI (2007-12-18). Assessment of mitomycin C-induced cytotoxicity and genotoxicity utilizing somatic progenies of human embryonic stem cells. ScholarBank@NUS Repository.
Abstract: Human embryonic stem cells (hESC) hold much potential in near future non-therapeutic applications. In this study, hESC somatic progenies of fibroblastic phenotype (designated pH9) were successfully differentiated from hESC cell line H9. We demonstrated that at passage 5, the euploid pH9 cells fully lost the pluripotency of hESC thus represents the physiology of non-genetic modified somatic cells. The potential applications of pH9 in cytotoxicity and genotoxicity testing were explored. Following a series of characterization, pH9 were treated with mitomycin C and the induced cytotoxicity and genotoxicity were evaluated by a battery of tests including MTT assay, PNA-FISH, micronucleus assay, cytokinesis-block micronucleus assay, micronucleus FISH and COMET assays. The results were compared with those obtained on traditional cell lines in ISO and OECD guidelines.
URI: http://scholarbank.nus.edu.sg/handle/10635/28132
Appears in Collections:Master's Theses (Open)

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