Demonstration of specific dust mite allergen-Induced response in a murine model

Abstract

Dust mite allergens are important triggers of atopic asthma. Native Der p 1 and recombinant Der p 2, Blo t 3, Blo t 5, Der p 7, Blo t 12, and Der f 13 were used to represent dust mite allergen groups of interest. Atopic sera reactivity assays and house dust sample screens were performed to profile the allergens in the local context. The intrinsic nature of the dust mite proteins and the allergen-host interaction response were investigated with a murine model of atopic asthma. Host immune responses to each allergen were measured by airway hyperresponsiveness (AHR), sera allergen-specific antibody profile, and lung histology. Results from the atopic population sera reactivity screens, the house dust distribution levels and the AHR responses demonstrated that each allergen group can exhibit differential immunological host responses and this may be attributed to the allergenb s intrinsic properties.