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https://scholarbank.nus.edu.sg/handle/10635/27872
DC Field | Value | |
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dc.title | Meat allergy and the allergenic components: Underlining reasons for the absence of clinical presentation to meat antigens despite the presence of high levels of specific IgE | |
dc.contributor.author | WONG KANG NING | |
dc.date.accessioned | 2011-10-18T18:02:38Z | |
dc.date.available | 2011-10-18T18:02:38Z | |
dc.date.issued | 2006-10-19 | |
dc.identifier.citation | WONG KANG NING (2006-10-19). Meat allergy and the allergenic components: Underlining reasons for the absence of clinical presentation to meat antigens despite the presence of high levels of specific IgE. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/27872 | |
dc.description.abstract | Little is known about meat allergy and the allergenic components involved. Using a dot-blot immunoarray, we showed high IgE-binding frequency to beef (8%), pork (12%) and mutton (5%) in 1096 allergic patientsa?? sera tested. High degree of cross-reactivity between the meat antigens was observed with inhibition ELISA. Identification and characterization of meat-based allergens were achieved using a dual bioinformatics (allergenicity prediction based on allergen-motif or sequence homology) and proteomics (2D electrophoresis and mass spectrometry) approach. Bioinformatics approach predicted 252 distinct putative allergens from six animal species whereas proteomics approach identified 56 IgE-reactive proteins from beef, pork and mutton. Despite presence of high levels of meat specific IgEs, only 2 out of 18 patients tested via SPT were beef-positive. The high IgE-binding to meat extracts is mainly due to presence of mammalian cross-reactive carbohydrate determinants (CCDs). Negative SPT is due to presence of a??blockinga?? IgG antibodies which inhibits histamine release. | |
dc.language.iso | en | |
dc.subject | meat, allergy, allergen, specific IgE, cross-reactive carbohydrate determinants (CCDs), specific IgG | |
dc.type | Thesis | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.supervisor | CHEW FOOK TIM | |
dc.description.degree | Master's | |
dc.description.degreeconferred | MASTER OF SCIENCE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Master's Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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WONG Kang Ning HT030973N Masters thesis .pdf | 4.11 MB | Adobe PDF | OPEN | None | View/Download |
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