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Title: Development of DNA vaccines for allergic Asthma
Keywords: allergy, asthma, Der p 1, DNA vaccine, optimization, evaluation
Issue Date: 21-Sep-2006
Source: HUANG FU TAO QI (2006-09-21). Development of DNA vaccines for allergic Asthma. ScholarBank@NUS Repository.
Abstract: Allergic asthma results from Th2 dominant immune responses to aeroallergens. Current treatments are mainly symptomatic. The aim of this study was to develop DNA vaccines for more effective management of this disease. Plasmid DNA containing the gene of mite allergen Der p 1 was constructed and tested in the mouse model. Expression of Der p 1 and its immunogenicity were confirmed after intramuscular injection of DNA construct. After further modifications, the optimized DNA construct was able to induce high magnitude Der p 1 specific Th1 skewed immune responses, which could subsequently prevent and treat allergic asthma in the mouse model. Using the same strategy, a DNA construct containing multiple allergen genes was also developed. In conclusion, this study developed a novel approach for prevention and treatment of allergic asthma. The optimization strategies employed in this study could be useful in the design of DNA vaccines in general.
Appears in Collections:Ph.D Theses (Open)

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