Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/27856
Title: Claudin-1 is the direct target of RUNX3 in gastric epithelial cells
Authors: CHANG TI LING
Keywords: RUNX3, tight junction, claudin-1, gastric cancer, TGF-beta
Issue Date: 24-Feb-2009
Source: CHANG TI LING (2009-02-24). Claudin-1 is the direct target of RUNX3 in gastric epithelial cells. ScholarBank@NUS Repository.
Abstract: The genes controlling cell-cell contact and cellular polarity are known to be heavily involved in cancer progression. Tumorigenic mouse GIF cells isolated from Runx3-/- gastric epithelium attached weakly to each other and did not form glandular structures on collagen gels as previously reported. In a search for RUNX3 target genes which play roles in gastric carcinogenesis, claudin-1 was found to be expressed at high level in Runx3+/+ gastric epithelial cells, but at very low level in Runx3-/- ones. TGF-o " induced expression of claudin-1 in SNU16, a human gastric cancer cell line. The TGF-o "-dependent expression of claudin-1, however, was not observed in RUNX3-knockdown SNU16 cells. In addition, hclaudin-1 promoter activity was up-regulated by the expression of RUNX3. The tumorigenicity of Runx3-/- GIF clones which stably expressed claudin-1 was significantly less than parental cell lines. Furthermore, claudin-1 and RUNX3 expression were found to be highly correlated in the human gastric cancers specimens. These results suggest the possible role of claudin-1 as a tumor suppressor, and its synergistic role with RUNX3 in the genesis of gastric cancer.
URI: http://scholarbank.nus.edu.sg/handle/10635/27856
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
PhD thesis.pdf2.01 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

232
checked on Dec 18, 2017

Download(s)

218
checked on Dec 18, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.