Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/27848
Title: Characterization of the function of tight junction proteins in transgenic mice
Authors: XU JIANLIANG
Keywords: ZO-1, ZO-2,ZO-3,tight junction, embryonic stem cells, embryoid bodies
Issue Date: 23-Dec-2008
Source: XU JIANLIANG (2008-12-23). Characterization of the function of tight junction proteins in transgenic mice. ScholarBank@NUS Repository.
Abstract: ZO-1, ZO-2 and ZO-3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as occludin, claudins and junctional adhesion molecules to the actin cytoskeleton. Despite being among the first TJ proteins to have been identified and having undergone extensive biochemical analysis, little is know about the physiological roles of individual ZO proteins in different tissues or during vertebrate development. Here, we show that ZO-3-/- mice lack an obvious phenotype. In contrast, embryos deficient for ZO-2 die shortly after implantation due to an arrest in early gastrulation. ZO-2-/- embryos show decreased proliferation at E6.5, increased apoptosis at E7.5 and altered architecture of the apical junctional complex as compared to wild-type. ZO-1-/- mice are currently unavailable, while chimeric mice derived from ZO-1-/- embryonic stem (ES) cells are embryonic lethal. Because of the embryonic lethality of the ZO-1-/- and ZO-2-/- mice, we also generated knockout ES cell lines. We have obtained ZO-1-/-, ZO-2-/-, ZO-3-/-, ZO-1-/-ZO-2-/-, and ZO-2-/-ZO-3-/- ES cells. These cell lines have shown various defects in their ability to differentiate into epithelial cells, cardiomyocytes or skeletal muscle cells.
URI: http://scholarbank.nus.edu.sg/handle/10635/27848
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