Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/27843
Title: Regulation of the TRIP-BR1 proto-oncoprotein - a potential therapeutic target for human cutaneous and intracavitory proliferative lesions
Authors: ZANG ZHIJIANG
Keywords: TRIP-Br1, PP2A, protein phosphatase, E2F, Neoplasm, chick embryo chorioallantoic membrane
Issue Date: 9-Apr-2008
Source: ZANG ZHIJIANG (2008-04-09). Regulation of the TRIP-BR1 proto-oncoprotein - a potential therapeutic target for human cutaneous and intracavitory proliferative lesions. ScholarBank@NUS Repository.
Abstract: TRIP-Br proteins are transcriptional regulators functioning at E2F1 promoter to integrate signals provided by PHD-bromodomain-containing proteins. Herein we demonstrated the synthetic decoy peptides competing with TRIP-Br1/2 for binding to PHD-bromodomain proteins elicited an anti-proliferative effect in representative human nasopharyngeal, cervical and melanoma cancer cells in vitro and in vivo. Serine/threonine protein phosphatase 2A (PP2A) BN1 was identified as a TRIP-Br-1 interactor. TRIP-Br1 is serine-phosphorylated. Inhibition of PP2A by okadaic acid or PP2A-C siRNA increased serine phosphorylated TRIP-Br1 and decreased total TRIP-Br1 protein level. Overexpression of the PP2A-C increased the total TRIP-Br1 protein and TRIP-Br1 co-activated of E2F1/DP1 transcription. Our data suggest antagonizing the integrator function of TRIP-Br may represent important chemotherapeutic drug target for superficial cutaneous and intracavitary hyperproliferative lesions and TRIP-Br1 protein level is positively regulates by PP2A. Uncovering the mechanism of TRIP-Br protein regulation may facilitate the application of therapeutic strategies targeting TRIP-Br proteins in human diseases.
URI: http://scholarbank.nus.edu.sg/handle/10635/27843
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