Please use this identifier to cite or link to this item:
Title: Cellular therapy of Diabetes Mellitus
Keywords: cellular gene therapy diabetes hepatocytes bone marrow mesenchymal stromal cells
Issue Date: 13-Mar-2009
Citation: CHEN KIN FOONG (2009-03-13). Cellular therapy of Diabetes Mellitus. ScholarBank@NUS Repository.
Abstract: Current gene- and cell-based therapies have significant limitations which impede clinical application. Taking diabetes mellitus as a paradigm, we show that ex vivo electrotransfer of glucose-responsive promoters-regulated nonviral insulin expression vectors in primary adult somatic cells i.e. murine and porcine hepatocytes and porcine bone marrow mesenchymal stem cells (PBMMSCs). Quasi-physiological glucose-regulated transgenic proinsulin transcription and insulin secretion was achieved in vitro. In two separate in vivo studies, immediate intrahepatic implantation of plasmid-electroporated syngeneic and autologous primary hepatocytes durably corrected murine and porcine streptozotocin (STZ)-induced diabetes, respectively. Importantly, fasting hypoglycaemia did not occur. Early correction of porcine diabetes ameliorated diabetes-associated metabolic derangements, tissue-level transcriptome changes and structural complications. In another in vivo experiment, xenotransplantation of modified PBMMSCs in NOD-scid mice ameliorated STZ-diabetes. In conclusion, this simple strategy is safe and effective, and may have more general application for the treatment of other diseases caused by specific protein deficiencies.
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Complete Thesis_Cellular Therapy of Diabetes Mellitus_Chen Kin Foong_Final uploaded.pdf20.85 MBAdobe PDF



Page view(s)

checked on Feb 3, 2019


checked on Feb 3, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.