Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/27791
Title: Construction of bacterial artificial chromosome library for Kineosphaera limosa strain Lpha5T and screening of genes involved in polyhydroxyalkanoate synthesis
Authors: JI ZHIJUAN
Keywords: Kineosphaera limosa strain Lpha5T, Bacterial Artificial Chromosome (BAC) library, Probe, Screening, Open Reading Frame (ORF), Gene Annotation
Issue Date: 26-Jan-2006
Source: JI ZHIJUAN (2006-01-26). Construction of bacterial artificial chromosome library for Kineosphaera limosa strain Lpha5T and screening of genes involved in polyhydroxyalkanoate synthesis. ScholarBank@NUS Repository.
Abstract: Bacterial artificial chromosome (BAC) library of Kineosphaera limosa strain Lpha5T was constructed. Lpha5T BAC library contains 7680 BAC clones with average insert of 23.5 kb. Screening of the library with probe specific for phaC gene, which was amplified using PCR from Alcoligenus Latus, allowed the identification of 18 positive BACs. A fragment hybridized with the probe was cloned and sequenced. The sequence in total contains 2186 bases. The distribution of ACGT along the strand was A: 14.18% (310 nt), T: 16.24% (355 nt), G: 41.03% (897 nt) and C: 28.45 % (622), which results in a GC content of 69.48%. Five open reading frames (ORFs) were found within the fragment. The number of nucleotide contained in each ORF was 84, 138, 507, 321 and 265 bp, encoding peptides with length of 27, 45, 168, 106 and 88 amino acids, respectively. The peptides have some important functions.
URI: http://scholarbank.nus.edu.sg/handle/10635/27791
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