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Title: Investigation of the regulatory network involving Agamous- Like 24 in floral transition of Arabidopsis
Keywords: Arabidopsis, Agmaous-like 24, floral transition
Issue Date: 11-Apr-2006
Source: WEN TIANFAN (2006-04-11). Investigation of the regulatory network involving Agamous- Like 24 in floral transition of Arabidopsis. ScholarBank@NUS Repository.
Abstract: Flowering is one of the most essential processes in the life cycle of a flowering plant. It requires a transition from vegetative to reproductive growth. A MADS-domain containing transcription factor, AGAMOUS-LIKE 24 (AGL24), plays an important role in integrating flowering time signals in Arabidopsis, but it is hitherto unknown what are the direct regulators upstream or downstream of AGL24. In this study, chromatin immunoprecipitation (ChIP) was applied to identify the downstream target genes of AGL24. Several putative target genes were identified by cloning and sequencing isolated DNA fragments in ChIP experiments. To study the molecular basis of the regulation of AGL24, the AGL24 promoter was isolated and this regulatory region was investigated by promoter deletion analysis. To facilitate our further studies of AGL24 target genes, an in situ hybridization system was established to examine the spatial and temporal expression of a specific gene.
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