Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/27769
Title: Specificity and diversity in the vertebrate nervous system: An analysis of two genes
Authors: MAHENDRA D. WAGLE
Keywords: vertebrate, zebrafish, ephrinb2, baculovirus, Rag1, chromatin immunoprecipitation, L1 retrotransposon
Issue Date: 23-Jan-2006
Source: MAHENDRA D. WAGLE (2006-01-23). Specificity and diversity in the vertebrate nervous system: An analysis of two genes. ScholarBank@NUS Repository.
Abstract: This thesis describes two genes that may establish different identities in neurons and thus mediate the formation of synaptic connections. The first gene, ephrinB2a, is expressed strongly in posterior zebrafish tectal neurons that are contacted by retinal axons. Ectopic expression of ephrinB2a in the anterior midbrain, with the aid of baculovirus, causes stalling of retinal axons. EphrinB2a may thus signal some retinal axons that they have reached their target neurons. The second gene, Rag1 (recombination activation gene-1), which mediates diversity in the immune system, is surprisingly also expressed in the vertebrate nervous system. Here, RAG1 protein is shown to be nuclear localized in a subset of differentiated mouse neurons. Chromatin immunoprecipitation, coupled with macroarray screening, identified a 5a?? repeat region in a LINE-1 retrotransposon, as a potential target of RAG1 in neurons. This raises the possibility that Rag1 may have a function in neurons by regulating a mobile element.
URI: http://scholarbank.nus.edu.sg/handle/10635/27769
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