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Title: Functional studies of BPGAP1, a novel BCH domain-containing RhoGAP protein
Authors: SHANG XUN
Keywords: Rho, RhoGAP, BCH, proline-rich, pseudopodia, cell dynamics
Issue Date: 31-May-2004
Citation: SHANG XUN (2004-05-31). Functional studies of BPGAP1, a novel BCH domain-containing RhoGAP protein. ScholarBank@NUS Repository.
Abstract: We have identified a novel RhoGAP, BPGAP1 (for BNIP-2 and Cdc42GAP Homology (BCH) domain-containing, Proline-rich and Cdc42GAP-like protein subtype-1), Which is ubiquitously expressed and shares 54% sequence identity to Cdc42GAP/p50RhoGAP. BPGAP1 selectively enhanced RhoA GTPase activity in vivo although it also interacted strongly with Cdc42 and Rac1. It formed complexes with other BCH domain-containing proteins. It induced pseudopodia and increased migration of MCF7 cells. Formation of pseudopodia required its BCH and GAP domains but not the proline-rich region, and was differentially inhibited by coexpression of constitutive active mutant of RhoA, or dominant negative mutants of Cdc42 and Rac1. However, mutant without the proline-rich region failed to confer any increase in cell migration despite the induction of pseudopodia. Our findings provide evidence that cell morphology changes and migration are coordinated via multiple domains in BPGAP1 and present a novel mode of regulation for cell dynamics by a RhoGAP protein.
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