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Title: Characterization of major and unique Blomia tropicalis mite allergens
Keywords: sensitization, rhinitis, asthmatic, Blo t 19, monoclonal, immunopurification
Issue Date: 27-Apr-2004
Citation: YEOH SHEAH MIN (2004-04-27). Characterization of major and unique Blomia tropicalis mite allergens. ScholarBank@NUS Repository.
Abstract: Sensitization profiles of rhinitis, non-rhinitis subjects and asthmatic subjects (from Singapore and Malaysia respectively) against three major mite allergens Der p 1, Der p 2 and Blo t 5 were studied using enzyme-linked immunosorbent assay (ELISA). It was shown that these allergens are important sensitizing agents and should be included in component-resolved diagnosis of mite sensitization. Besides that, a unique allergen from Blomia tropicalis (Blo t), Blo t 19 was identified through cDNA library screening. Blo t 19 is a small (around 7 kD) and cysteine-rich protein. Recombinant form of Blo t 19 was a minor allergen. A Blo t 19-specifc monoclonal antibody which was useful for detection of Blo t 19 in western blot and ELISA was successfully raised using a combination of DNA immunization and protein boost in mice. However, the purification procedures of native Blo t 19 using this monoclonal antibody remain elusive.
Appears in Collections:Master's Theses (Open)

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