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Title: Application of Conjugated Polyelectrolyte in Biosensor
Authors: PU KANYI
Keywords: conjugated polyelectrolyte, energy transfer, biosensor, DNA, protein, cell imaging
Issue Date: 1-Dec-2010
Citation: PU KANYI (2010-12-01). Application of Conjugated Polyelectrolyte in Biosensor. ScholarBank@NUS Repository.
Abstract: Reliable technologies for the detection of chemical and biological substances are of scientific importance and economic interest because of their vital applications in clinical diagnosis, environmental monitoring, forensic analysis and antiterrorism. In this regard, conjugated polyelectrolytes (CPEs) with electron-delocalized fluorescent backbones and water-soluble ionic side chains have provided a unique platform for the construction of biosensors. However, fast, simple and label-free visual sensing strategies remain lacking for CPE-based biosensors. In this doctoral dissertation work, a series of new CPEs are designed and synthesized to constitute effective fluorescence resonance energy transfer (FRET) probes for label-free visual detection of physiologically important biomolecules such as heparin, double-stranded DNA (dsDNA), and proteins. Two kinds of FRET probes are developed, which include the CPEs with intramolecular energy donor-acceptor backbones (single-component systems) and the CPE blends with energy donor-acceptor pair (bicomponent systems). Both kinds of CPE-based probes change the fluorescent colors upon interacting with the targets of interest due to enhanced FRET, consequently allowing for in-situ visual detection. In addition to solution-state sensing, efficient FRET is observed in cell for the CPE derivative with a unimolecular nanoarchitecture, which paves the way for the development of intracellular assays. The label-free visual biosensors developed herein together with the underlying mechanisms unraveled thereof should provide useful guidelines for the advance of CPEs in biological applications.
Appears in Collections:Ph.D Theses (Open)

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