Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.neuint.2006.11.008
Title: Upregulation of dihydropyrimidinase-related protein 2, spectrin α II chain, heat shock cognate protein 70 pseudogene 1 and tropomodulin 2 after focal cerebral ischemia in rats-A proteomics approach
Authors: Chen, A.
Liao, W.P. 
Lu, Q.
Wong, W.S.F. 
Wong, P.T.-H. 
Keywords: Cerebral ischemia
Protein expression
Proteome
Stroke
Issue Date: 2007
Source: Chen, A., Liao, W.P., Lu, Q., Wong, W.S.F., Wong, P.T.-H. (2007). Upregulation of dihydropyrimidinase-related protein 2, spectrin α II chain, heat shock cognate protein 70 pseudogene 1 and tropomodulin 2 after focal cerebral ischemia in rats-A proteomics approach. Neurochemistry International 50 (7-8) : 1078-1086. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neuint.2006.11.008
Abstract: In recent years, there are an increasing number of proteomics studies that investigated the alterations in the protein expression relevant to human diseases but none for stroke. We, therefore, attempted such a study in a paradigm of focal cerebral ischemia in rat. Rats were subjected to cerebral ischemia by unilateral occlusion of the middle cerebral artery. Global protein analysis was performed after 24 h on the lesioned and sham-control cerebral cortex using two-dimensional gel electrophoresis. Protein spots with more than a 3-fold change in intensity were identified by mass spectrometry. Middle cerebral artery occlusion (MCAO) caused infarct volume of 18-22% predominantly in the cortex of the lesioned hemisphere. Two-dimensional gel electrophoresis resolved about 1500 protein spots of which only 12 were significantly upregulated by 3-46-fold. Three spots were identified to be dihydropyrimidinase-related protein 2 (DRP-2, also known as collapsin response mediator protein 2 (CRMP-2) or turned on after division, 64 kD protein (TOAD-64)). The spots varied in pI values only and this may reflect different phosphorylation status of the same protein. Two spots were identified as spectrin α II chain (rat fragment, also known as α-fodrin or non-erythroid α chain, SPNA-2); and one spot each for heat shock cognate protein 70 pseudogene 1 (HSC70-ps1, also known as heat shock protein 8 pseudogene 1), and tropomodulin 2 (Tmod2). The upregulation of protein expression was corroborated by observed upregulation of mRNA expression. The remaining five spots were not identified satisfactorily. As DRP-2, spectrin, and Tmod2 are involved in axonal and neurite growth as well as synaptic plasticity and maturation, the presently observed upregulation of the expression of these proteins may indicate active neuroregeneration and repair at 24 h after the induction of cerebral ischemia. © 2006 Elsevier Ltd. All rights reserved.
Source Title: Neurochemistry International
URI: http://scholarbank.nus.edu.sg/handle/10635/27215
ISSN: 01970186
DOI: 10.1016/j.neuint.2006.11.008
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

75
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

72
checked on Nov 23, 2017

Page view(s)

150
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.