Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jpba.2008.12.036
Title: Determination of breviflavone A and B in Epimedium herbs with liquid chromatography-tandem mass spectrometry
Authors: Hong, X. 
Wang, X.
Yong, E.L. 
Gong, Y. 
Keywords: Breviflavone
Epimedium
Liquid chromatography tandem mass spectrometry
Phytoestrogen
Issue Date: 2009
Source: Hong, X., Wang, X., Yong, E.L., Gong, Y. (2009). Determination of breviflavone A and B in Epimedium herbs with liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis 49 (3) : 853-857. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jpba.2008.12.036
Abstract: Two new types of minor flavonoids, breviflavone A and B, have been recently isolated and identified from Epimedium brevicornu in our previous research. Breviflavone B is a novel flavonoid with potent and specific estrogen receptor (ER) bioactivity. Its positional isomer, breviflavone A, is not ER active. Therefore, it is important to determine the two minor components, breviflavone A and B, in Epimedium herbs. In this report, a robust method for measurement of the two breviflavones in Epimedium ethanolic extracts has been developed by using liquid chromatography tandem mass spectrometry via selected-reaction monitoring (m/z 437 → m/z 367 for breviflavone A and m/z 437 → m/z 351 for breviflavone B) under negative electrospray ionization mode. This method has been successfully used to determine the two breviflavones in ethanolic herbal extracts of five major Epimedium species (E. brevicornu, E. koreanum, E. pubescens, E. sagittatum, and E. wushanese) from various sources. The contents of the two breviflavones range from 0.0181 to 0.1791% for breviflavone A and 0.0026 to 0.0252% for breviflavone B in the dried ethanolic extracts of those Epimedium herbal samples. © 2009 Elsevier B.V. All rights reserved.
Source Title: Journal of Pharmaceutical and Biomedical Analysis
URI: http://scholarbank.nus.edu.sg/handle/10635/26023
ISSN: 07317085
DOI: 10.1016/j.jpba.2008.12.036
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