Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/25821
Title: Characterization of Liver X receptor- and retinoid acid receptor-mediated response with transcriptome and histological analysis in zebrafish liver
Authors: HENDRIAN SUKARDI
Keywords: zebrafish, liver x receptor, lxr, transcriptome, omics, microarrays
Issue Date: 22-Dec-2010
Source: HENDRIAN SUKARDI (2010-12-22). Characterization of Liver X receptor- and retinoid acid receptor-mediated response with transcriptome and histological analysis in zebrafish liver. ScholarBank@NUS Repository.
Abstract: Nuclear receptor, a class of ligand-activated transcription factor, regulates many important physiological processes. Therefore nuclear receptors, such as liver x receptor (LXR) and retinoic acid receptor (RAR), are attractive therapeutic targets. Although the zebrafish is a prominent vertebrate model that has recently gained surging interest for disease modeling and drug screening, currently little is known with regards to LXR- and RAR-induced responses in zebrafish liver. In our efforts to investigate the potential of zebrafish as a model for LXR- and RAR-related studies, we performed experiments using adult male zebrafish exposed to all-trans retinoic acid (RAR agonist) or T0901317 (LXR agonist) for 96 hours before sampling the liver for histological, transcriptomic and realtime PCR analyses. We observed LXR and RAR activation modulate several biological processes involved in immune system and metabolic processes. Our transcriptomic analysis corroborated with our histological analysis and real-time PCR analysis. We were able to capture known effects of LXR and RAR activation as reported in mammalian models, suggesting conserved mode-of-actions between mammals and fish. Our findings indicate that zebrafish is a valid model for investigating LXR and RAR drug targets, LXR- and RAR-mediated disruptions and metabolic disorders.
URI: http://scholarbank.nus.edu.sg/handle/10635/25821
Appears in Collections:Master's Theses (Open)

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