Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jbiotec.2010.03.018
Title: Metabolomics-driven approach for the improvement of Chinese hamster ovary cell growth: Overexpression of malate dehydrogenase II
Authors: Chong, W.P.K.
Reddy, S.G.
Yusufi, F.N.K.
Lee, D.-Y. 
Wong, N.S.C.
Yap, M.G.S.
Ho, Y.S.
Heng, C.K. 
Keywords: Aspartate
Chinese hamster ovary
Liquid chromatography-mass spectrometry (LC-MS)
Malate dehydrogenase II
Metabolic engineering
Metabolomics
Issue Date: 2010
Source: Chong, W.P.K., Reddy, S.G., Yusufi, F.N.K., Lee, D.-Y., Wong, N.S.C., Yap, M.G.S., Ho, Y.S., Heng, C.K. (2010). Metabolomics-driven approach for the improvement of Chinese hamster ovary cell growth: Overexpression of malate dehydrogenase II. Journal of Biotechnology 147 (2) : 116-121. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jbiotec.2010.03.018
Abstract: We have established a liquid chromatography-mass spectrometry based metabolomics platform to identify extracellular metabolites in the medium of recombinant Chinese hamster ovary (CHO) fed-batch reactor cultures. Amongst the extracellular metabolites identified, malate accumulation was the most significant. The contributing factors to malate efflux were found to be the supply of aspartate from the medium, and an enzymatic bottleneck at malate dehydrogenase II (MDH II) in the tricarboxylic acid cycle. Subsequent metabolic engineering to overexpress MDH II in CHO resulted in increases in intracellular ATP and NADH, and up to 1.9-fold improvement in integral viable cell number. © 2010 Elsevier B.V.
Source Title: Journal of Biotechnology
URI: http://scholarbank.nus.edu.sg/handle/10635/25773
ISSN: 01681656
DOI: 10.1016/j.jbiotec.2010.03.018
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