Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.mcp.2005.06.008
Title: Rapid detection of codon 460 mutations in the UL97 gene of ganciclovir-resistant cytomegalovirus clinical isolates by real-time PCR using molecular beacons
Authors: Yeo, A.C.
Yap, H.K. 
Chan, K.P.
Kumarasinghe, G.
Keywords: Cytomegalovirus
Molecular beacons
Real-time PCR
Issue Date: 2005
Source: Yeo, A.C., Yap, H.K., Chan, K.P., Kumarasinghe, G. (2005). Rapid detection of codon 460 mutations in the UL97 gene of ganciclovir-resistant cytomegalovirus clinical isolates by real-time PCR using molecular beacons. Molecular and Cellular Probes 19 (6) : 389-393. ScholarBank@NUS Repository. https://doi.org/10.1016/j.mcp.2005.06.008
Abstract: A rapid real-time polymerase chain reaction (PCR) assay using molecular beacons has been developed for the simultaneous detection of wild-type and mutant strains of cytomegaloviruses (CMV) with respect to codon 460 of the UL97 gene has been developed. The molecular beacons were designed to complement the wild-type codon 460 or the mutant sequence arising from a single base-pair difference (point mutation). Discrimination between wild-type and mutant templates was demonstrated as the beacons did not generate fluorescence with their respective mismatch targets but only with those that they were designed to perfectly anneal with. Samples that harbor mixed populations of CMV could also be readily recognized. Applied to a small number of clinical samples, the retrospective screening by this assay are in general concordance with that obtained by PCR-RFLP. Using molecular beacons strategy, codon 460 mutation was detected in ten out o the total number of 40 samples, whereas the latter method identified nine samples as containing the mutation. The discrepant result arose from the genotyping of one clinical sample as mixed (containing both wild-type and mutant CMV strains) by molecular beacons but as wild-type by PCR-RFLP, suggesting that this real-time strategy is possibly more sensitive for mutation analysis. © 2005 Elsevier Ltd. All rights reserved.
Source Title: Molecular and Cellular Probes
URI: http://scholarbank.nus.edu.sg/handle/10635/25690
ISSN: 08908508
DOI: 10.1016/j.mcp.2005.06.008
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

21
checked on Dec 11, 2017

WEB OF SCIENCETM
Citations

16
checked on Dec 11, 2017

Page view(s)

129
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.