Please use this identifier to cite or link to this item:
|Title:||Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection|
Dissociation curve analysis
|Citation:||Quek, D.L., Ng, Y.-Y., Tang-Lim, G.-I., Wang, W., Chong, S.S., Tan, A.S.C., Ng, I.S.L., Law, H.-Y. (2007). Rapid carrier screening for β-thalassemia by single-step allele-specific PCR and detection. Clinical Biochemistry 40 (5-6) : 427-430. ScholarBank@NUS Repository. https://doi.org/10.1016/j.clinbiochem.2007.01.003|
|Abstract:||Objectives: This study aimed to evaluate a rapid molecular carrier screening strategy for β-thalassemia. Design and methods: Allele-specific PCR was combined with amplicon detection by dissociation curve analysis of SYBR® Green I fluorescence in a single step. Results: The presence of a particular mutation results in the amplification of a mutation-specific product and the dissociation temperature of each amplicon was highly reproducible. Conclusions: Homogeneous allele-specific PCR amplification and detection of multiple β-globin mutations can serve as a rapid and inexpensive carrier screening tool. © 2007 The Canadian Society of Clinical Chemists.|
|Source Title:||Clinical Biochemistry|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 11, 2018
WEB OF SCIENCETM
checked on Oct 2, 2018
checked on Oct 14, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.