Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2010.01.081
Title: Biodegradable magnetic-fluorescent magnetite/poly(dl-lactic acid-co-α,β-malic acid) composite nanoparticles for stem cell labeling
Authors: Wang, L. 
Shuter, B. 
Neoh, K.-G. 
Kang, E.-T. 
Wang, S.-C. 
Keywords: Biodegradable
MRI
Nanoparticles
Poly(dl-lactic acid-co-α,β-malic acid)
Stem cells
Issue Date: 2010
Citation: Wang, L., Shuter, B., Neoh, K.-G., Kang, E.-T., Wang, S.-C. (2010). Biodegradable magnetic-fluorescent magnetite/poly(dl-lactic acid-co-α,β-malic acid) composite nanoparticles for stem cell labeling. Biomaterials 31 (13) : 3502-3511. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.01.081
Abstract: Bifunctional superparamagnetic magnetite/poly(dl-lactic acid-co-α,β-malic acid) composite nanoparticles (PLMA-MNPs) detectable by both magnetic resonance imaging (MRI) and fluorescence microscopy were synthesized by coating Fe3O4 nanoparticles with biodegradable poly(dl-lactic acid-co-α,β-malic acid) copolymer (PLMA) with covalently bound fluorescein isothiocyanate (FITC). The FITC modified PLMA-MNPs (FITC-PLMA-MNPs) have a hydrodynamic diameter of 100 nm and an anionic surface. MTT assays of mouse macrophages, 3T3 fibroblasts and human mesenchymal stem cells (hMSCs) incubated with these nanoparticles indicated that these nanoparticles did not possess significant cytotoxicity. Furthermore, the osteogenic and adipogenic differentiations of the hMSCs were not influenced by the labeling process. As a result of the high R2 (164.8 mm-1 s-1) and R2/R1 ratio (32) of the FITC-PLMA-MNPs, the labeled hMSCs can be detected by a clinical 3T MRI scanner at an in vitro detection threshold of about 1200 cells. The green fluorescence associated with the FITC can be readily observed. Such nanoparticles can potentially be used as a T2-weighted contrast agent and fluorescent agent for stem cell labeling. © 2010 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/25575
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2010.01.081
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

89
checked on Dec 5, 2018

WEB OF SCIENCETM
Citations

87
checked on Nov 27, 2018

Page view(s)

293
checked on Oct 14, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.