Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/25401
Title: OVEREXPRESSION OF TYRO3 AND ITS IMPLICATION ON HEPATOCELLULAR CARCINOMA (HCC) PROGRESSION
Authors: DUAN YAN
Keywords: Tyro3, tyrosine kinase, hepatocellular carcinoma, alpha-fetoprotein, cell proliferation
Issue Date: 26-Apr-2011
Source: DUAN YAN (2011-04-26). OVEREXPRESSION OF TYRO3 AND ITS IMPLICATION ON HEPATOCELLULAR CARCINOMA (HCC) PROGRESSION. ScholarBank@NUS Repository.
Abstract: The lack of effective treatment against hepatocellular carcinoma (HCC), the fifth most common malignancy and the third leading cause of cancer deaths worldwide calls for direct efforts to better understand the disease and identify new drug targets. In the search for novel multi-kinase inhibitors to treat this disease, our group found a very potent compound which acts on a relatively uncharacterized receptor tyrosine kinase, Tyro3. To explore the potential role of tyrosine kinase Tyro3 in HCC, we examined the expression of Tyro3 in HCC tumors and correlated it with clinical outcomes. Using cDNAs derived from 56 HCC patients and quantitative RT-PCR (qRT-PCR) analysis of Tyro3, we found frequent and significant overexpression which also corresponded to elevation of clinico-pathological markers for HCC such as hepatitis B virus (HBV) infection, a-fetoprotein (AFP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. To determine the cause-and-effect relationship between Tyro3 expression and these HCC phenotypes, we performed in vitro investigation using siRNA silencing of Tyro3 in a high expressing HCC cell line, Hep3B and found it to suppress cell proliferation. From these efforts, we have gathered important basis for further work to characterize Tyro3 as a potential and novel drug target in HCC. Of equal importance, we have successfully developed useful and relevant in vitro models that will support subsequent effort to understand the exact mechanism behind its effect.
URI: http://scholarbank.nus.edu.sg/handle/10635/25401
Appears in Collections:Master's Theses (Open)

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