Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2008.04.012
Title: In vivo study of anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold
Authors: Fan, H. 
Liu, H. 
Wong, E.J.W.
Goh, J.C.H. 
Toh, S.L.
Keywords: Ligament
Mesenchymal stem cells
Regeneration
Silk
Tissue engineering
Issue Date: 2008
Source: Fan, H., Liu, H., Wong, E.J.W., Goh, J.C.H., Toh, S.L. (2008). In vivo study of anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold. Biomaterials 29 (23) : 3324-3337. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2008.04.012
Abstract: Although most in vitro studies indicate that silk is a suitable biomaterial for ligament tissue engineering, in vivo studies of implanted silk scaffolds for ligament reconstruction are still lacking. The objective of this study is to investigate anterior cruciate ligament (ACL) regeneration using mesenchymal stem cells (MSCs) and silk scaffold. The scaffold was fabricated by incorporating microporous silk sponges into knitted silk mesh, which mimicked the structures of ligament extracellular matrix (ECM). In vitro culture demonstrated that MSCs on scaffolds proliferated vigorously and produced abundant collagen. The transcription levels of ligament-specific genes also increased with time. Then MSCs/scaffold was implanted to regenerate ACL in vivo. After 24 weeks, histology observation showed that MSCs were distributed throughout the regenerated ligament and exhibited fibroblast morphology. The key ligament ECM components including collagen I, collagen III, and tenascin-C were produced prominently. Furthermore, direct ligament-bone insertion with typical four zones (bone, mineralized fibrocartilage, fibrocartilage, ligament) was reconstructed, which resembled the native structure of ACL-bone insertion. The tensile strength of regenerated ligament also met the mechanical requirements. Moreover, its histological grading score was significantly higher than that of control. In conclusion, the results imply that silk scaffold has great potentials in future clinical applications. © 2008 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/25287
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2008.04.012
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