Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2010.06.020
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dc.titleRapid construction of mechanically- confined multi- cellular structures using dendrimeric intercellular linker
dc.contributor.authorMo, X.
dc.contributor.authorHong, Tan C.
dc.contributor.authorLi, Q.
dc.contributor.authorZheng, B.
dc.contributor.authorKang, C.H.
dc.contributor.authorNugraha, B.
dc.contributor.authorYue, Z.
dc.contributor.authorChoudhury, D.
dc.contributor.authorArooz, T.
dc.contributor.authorYu, H.
dc.contributor.authorLim, C.T.
dc.contributor.authorYi, Lui L.W.
dc.contributor.authorJia, R.R.
dc.contributor.authorShen, S.
dc.contributor.authorFu, H.X.
dc.contributor.authorYan, J.
dc.date.accessioned2011-08-01T03:06:20Z
dc.date.available2011-08-01T03:06:20Z
dc.date.issued2010
dc.identifier.citationMo, X., Hong, Tan C., Li, Q., Zheng, B., Kang, C.H., Nugraha, B., Yue, Z., Choudhury, D., Arooz, T., Yu, H., Lim, C.T., Yi, Lui L.W., Jia, R.R., Shen, S., Fu, H.X., Yan, J. (2010). Rapid construction of mechanically- confined multi- cellular structures using dendrimeric intercellular linker. Biomaterials 31 (29) : 7455-7467  . ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.06.020
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25179
dc.description.abstractTissue constructs that mimic the in vivo cell-cell and cell-matrix interactions are especially useful for applications involving the cell- dense and matrix- poor internal organs. Rapid and precise arrangement of cells into functional tissue constructs remains a challenge in tissue engineering. We demonstrate rapid assembly of C3A cells into multi- cell structures using a dendrimeric intercellular linker. The linker is composed of oleyl- polyethylene glycol (PEG) derivatives conjugated to a 16 arms- polypropylenimine hexadecaamine (DAB) dendrimer. The positively charged multivalent dendrimer concentrates the linker onto the negatively charged cell surface to facilitate efficient insertion of the hydrophobic oleyl groups into the cellular membrane. Bringing linker- treated cells into close proximity to each other via mechanical means such as centrifugation and micromanipulation enables their rapid assembly into multi- cellular structures within minutes. The cells exhibit high levels of viability, proliferation, three- dimensional (3D) cell morphology and other functions in the constructs. We constructed defined multi- cellular structures such as rings, sheets or branching rods that can serve as potential tissue building blocks to be further assembled into complex 3D tissue constructs for biomedical applications. © 2010 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2010.06.020
dc.sourceScopus
dc.subjectCell- ECM interaction
dc.subjectDefined cellular structures
dc.subjectMulticellular structures
dc.subjectOleyl poly (ethylene glycol) (oleyl-PEG)
dc.subjectRapid dendrimer
dc.subjectTissue engineering
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentPHYSICS
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentMECHANICAL ENGINEERING
dc.description.doi10.1016/j.biomaterials.2010.06.020
dc.description.sourcetitleBiomaterials
dc.description.volume31
dc.description.issue29
dc.description.page7455-7467  
dc.identifier.isiut000281183200005
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