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Title: Synthetic sandwich culture of 3D hepatocyte monolayer
Authors: Du, Y.
Han, R.
Wen, F. 
Ng, San San S.
Xia, L.
Leo, H.L.
Yu, H. 
Wohland, T. 
Keywords: Galactosylation
RGD peptide
Sandwich culture
Synthetic materials
Issue Date: 2008
Citation: Du, Y., Han, R., Wen, F., Ng, San San S., Xia, L., Leo, H.L., Yu, H., Wohland, T. (2008). Synthetic sandwich culture of 3D hepatocyte monolayer. Biomaterials 29 (3) : 290-301. ScholarBank@NUS Repository.
Abstract: The sandwich culture of hepatocytes, between double layers of extra-cellular matrix (ECM), is a well-established in vitro model for re-establishing hepatic polarity and maintaining differentiated functions. Applications of the ECM-based sandwich culture are limited by the mass transfer barriers induced by the top gelled ECM layer, complex molecular composition of ECM with batch-to-batch variation and uncontrollable coating of the ECM double layers. We have addressed these limitations of the ECM-based sandwich culture by developing an 'ECM-free' synthetic sandwich culture, which is constructed by sandwiching a 3D hepatocyte monolayer between a glycine-arginine-glycine-aspatic acid-serine (GRGDS)-modified polyethylene terephthalate (PET) track-etched membrane (top support) and a galactosylated PET film (bottom substratum). The bioactive top support and bottom substratum in the synthetic sandwich culture substituted for the functionalities of the ECM in the ECM-based sandwich culture with further improvement in mass transfer and optimal material properties. The 3D hepatocyte monolayer in the synthetic sandwich culture exhibited a similar process of hepatic polarity formation, better cell-cell interaction and improved differentiated functions over 14-day culture compared to the hepatocytes in collagen sandwich culture. The novel 3D hepatocyte monolayer sandwich culture using bioactive synthetic materials may readily replace the ECM-based sandwich culture for liver tissue engineering applications, such as drug metabolism/toxicity testing and hepatocyte-based bioreactors. © 2007 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2007.09.016
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