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Title: Transient inter-cellular polymeric linker
Authors: Ong, S.-M.
Arooz, T.
Tang, G.
Yu, H. 
He, L.
Tan, C.-H. 
Thuy, Linh N.T.
Tee, Y.-H.
Keywords: Cell aggregates
Cell-ECM interaction
Collagen assay
Organ printing
Tissue engineering
Issue Date: 2007
Citation: Ong, S.-M., Arooz, T., Tang, G., Yu, H., He, L., Tan, C.-H., Thuy, Linh N.T., Tee, Y.-H. (2007). Transient inter-cellular polymeric linker. Biomaterials 28 (25) : 3656-3667. ScholarBank@NUS Repository.
Abstract: Three-dimensional (3D) tissue-engineered constructs with bio-mimicry cell-cell and cell-matrix interactions are useful in regenerative medicine. In cell-dense and matrix-poor tissues of the internal organs, cells support one another via cell-cell interactions, supplemented by small amount of the extra-cellular matrices (ECM) secreted by the cells. Here we connect HepG2 cells directly but transiently with inter-cellular polymeric linker to facilitate cell-cell interaction and aggregation. The linker consists of a non-toxic low molecular-weight polyethyleneimine (PEI) backbone conjugated with multiple hydrazide groups that can aggregate cells within 30 min by reacting with the aldehyde handles on the chemically modified cell-surface glycoproteins. The cells in the cellular aggregates proliferated; and maintained the cortical actin distribution of the 3D cell morphology while non-aggregated cells died over 7 days of suspension culture. The aggregates lost distinguishable cell-cell boundaries within 3 days; and the ECM fibers became visible around cells from day 3 onwards while the inter-cellular polymeric linker disappeared from the cell surfaces over time. The transient inter-cellular polymeric linker can be useful for forming 3D cellular and tissue constructs without bulk biomaterials or extensive network of engineered ECM for various applications. © 2007 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2007.04.034
Appears in Collections:Staff Publications

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