Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2006.07.018
Title: 3D hepatocyte monolayer on hybrid RGD/galactose substratum
Authors: Du, Y.
Han, R.
Yu, H. 
Chia, S.-m. 
Chang, S. 
Tang, H.
Keywords: Galactosylation
Hepatocyte
Hepatotoxicity
Hybrid biomaterial
RGD peptide
Surface modification
Issue Date: 2006
Source: Du, Y., Han, R., Yu, H., Chia, S.-m., Chang, S., Tang, H. (2006). 3D hepatocyte monolayer on hybrid RGD/galactose substratum. Biomaterials 27 (33) : 5669-5680. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2006.07.018
Abstract: Hepatocyte-based applications such as xenobiotics metabolism and toxicity studies usually require hepatocytes anchoring onto flat substrata that support their functional maintenance. Conventional cell culture plates coated with natural matrices or synthetic ligands allow hepatocytes to adhere tightly as two-dimensional (2D) monolayer but these tightly anchored hepatocytes rapidly lose their differentiated functions. On galactosylated substrata, hepatocytes adhere loosely; and readily form three-dimensional (3D) spheroids that can maintain high levels of cellular functions. These spheroids detach easily from the substrata and exhibit poor mass transport properties unsuitable for many applications. Here, we have developed a hybrid RGD/galactose substratum based on polyethylene terephthalate film conjugated with both RGD peptide and galactose ligand to enhance cell adhesion and functions synergistically. Primary hepatocytes adhere effectively onto the transparent hybrid substratum in 96-well plates as monolayer while exhibiting high levels of liver-specific functions, morphology and cell-cell interactions typically seen in the 3D hepatocyte spheroids. The hepatocytes cultured onto the hybrid substratum also exhibit high levels of sensitivity to a model drug acetaminophen similar to the 3D hepatocyte spheroids. The monolayer of hepatocytes exhibiting the 3D cell behaviors on this flat hybrid substratum can be useful for various applications requiring both effective mass transfer and cellular support. © 2006 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/24983
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2006.07.018
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

68
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

64
checked on Nov 29, 2017

Page view(s)

252
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.