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|Title:||The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system|
|Authors:||Zhang, C. |
van, Noort D.
|Keywords:||3D cell constructs|
Transforming growth factor
|Citation:||Zhang, C., Ong, S.-M., Zhang, S., Toh, Y.-C., van, Noort D., Yu, H., Chia, S.-M. (2009). The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system. Biomaterials 30 (23-24) : 3847-3853. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2009.03.052|
|Abstract:||3D-microfluidic cell culture systems (3D-μFCCSs) support hepatocyte functions in vitro which can be further enhanced by controlled presentation of 100-200 pg/ml TGF-β1, thus mimicking the roles of supporting cells in co-cultures. Controlled presentation of TGF-β1 is achieved by either direct perfusion or in situ controlled release from gelatin microspheres immobilized in the 3D-μFCCS. Primary hepatocytes cultured for 7 days with the in situ controlled released TGF-β1 exhibited up to four-fold higher albumin secretion and two-fold higher phase I/II enzymatic activities, significantly improving the sensitivity of hepatocytes to acetaminophen-mediated hepatotoxicity, compared to hepatocytes cultured with directly perfused TGF-β1 or without TGF-β1. The controlled presentation of TGF-β1 enhanced hepatocyte functions in microfluidic systems without the complications of co-cultures, allowing for simplifications in drug testing and other hepatocyte-based applications. © 2009 Elsevier Ltd. All rights reserved.|
|Appears in Collections:||Staff Publications|
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