Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.imbio.2006.12.001
Title: The SARS coronavirus spike glycoprotein is selectively recognized by lung surfactant protein D and activates macrophages
Authors: Leth-Larsen, R. 
Zhong, F. 
Chow, V.T.K. 
Lu, J. 
Holmskov, U.
Keywords: Dendritic cells
Macrophages
MBL
SARS-CoV
SP-A
SP-D
Issue Date: 2007
Source: Leth-Larsen, R., Zhong, F., Chow, V.T.K., Lu, J., Holmskov, U. (2007). The SARS coronavirus spike glycoprotein is selectively recognized by lung surfactant protein D and activates macrophages. Immunobiology 212 (3) : 201-211. ScholarBank@NUS Repository. https://doi.org/10.1016/j.imbio.2006.12.001
Abstract: The severe acute respiratory syndrome coronavirus (SARS-CoV) infects host cells with its surface glycosylated spike-protein (S-protein). Here we expressed the SARS-CoV S-protein to investigate its interactions with innate immune mechanisms in the lung. The purified S-protein was detected as a 210 kDa glycosylated protein. It was not secreted in the presence of tunicamycin and was detected as a 130 kDa protein in the cell lysate. The purified S-protein bound to Vero but not 293T cells and was itself recognized by lung surfactant protein D (SP-D), a collectin found in the lung alveoli. The binding required Ca2+ and was inhibited by maltose. The serum collectin, mannan-binding lectin (MBL), exhibited no detectable binding to the purified S-protein. S-protein binds and activates macrophages but not dendritic cells (DCs). It suggests that SARS-CoV interacts with innate immune mechanisms in the lung through its S-protein and regulates pulmonary inflammation. © 2006 Elsevier GmbH. All rights reserved.
Source Title: Immunobiology
URI: http://scholarbank.nus.edu.sg/handle/10635/24861
ISSN: 01712985
DOI: 10.1016/j.imbio.2006.12.001
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

16
checked on Apr 18, 2018

WEB OF SCIENCETM
Citations

13
checked on Apr 18, 2018

Page view(s)

227
checked on Apr 21, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.