Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.atherosclerosis.2005.04.019
Title: Expression of GM3 synthase in human atherosclerotic lesions
Authors: Bobryshev, Y.V. 
Tran, D.
Lord, R.S.A.
Golovanova, N.K.
Samovilova, N.N.
Gracheva, E.V.
Efremov, E.E.
Prokazova, N.V.
Sobolev, A.Y.
Yurchenko, Y.V.
Cao, W. 
Lu, J. 
Saito, M.
Keywords: Arteries
Atherosclerosis
Dendritic cells
Ganglioside GM3
GM3 synthase
Macrophages
Issue Date: 2006
Source: Bobryshev, Y.V., Tran, D., Lord, R.S.A., Golovanova, N.K., Samovilova, N.N., Gracheva, E.V., Efremov, E.E., Prokazova, N.V., Sobolev, A.Y., Yurchenko, Y.V., Cao, W., Lu, J., Saito, M. (2006). Expression of GM3 synthase in human atherosclerotic lesions. Atherosclerosis 184 (1) : 63-71. ScholarBank@NUS Repository. https://doi.org/10.1016/j.atherosclerosis.2005.04.019
Abstract: We have previously demonstrated that amounts of ganglioside GM3 are markedly higher in human atherosclerotic lesions compared to that in non-diseased arterial tissue. Because the fatty acid composition of GM3 in blood plasma low density lipoproteins (LDL) and the fatty acid composition of GM3 in atherosclerotic lesions differed, we hypothesized that, in addition to GM3 originating from LDL infiltrating the arterial wall from the blood, excessive GM3 may be synthesized locally in atherosclerotic lesions. In the present work, using an anti-GM3 antibody developed by us, we showed that the levels of GM3 synthase in membrane fractions isolated from the atherosclerotic intima were higher compared to those in non-diseased arterial tissue. Using an immunohistochemical approach, we examined the expression of GM3 synthase in sections of atherosclerotic plaques and non-diseased arterial wall. GM3 synthase immunopositivity was found to be low in non-diseased arterial intima but large numbers of GM3 synthase-immunopositive cells were observed in atherosclerotic plaques. GM3 synthase was overexpressed by macrophages and dendritic cells and double immunostaining demonstrated cellular co-localization of GM3 synthase and GM3. Further in vitro experiments showed that both monocyte-derived dendritic cells and macrophages expressed high levels of GM3 synthase. The findings of the present study indicate that, at least partially, excessive amounts of GM3 in atherosclerotic lesions can be synthesized by macrophages and dendritic cells directly within the arterial wall. © 2005 Elsevier Ireland Ltd. All rights reserved.
Source Title: Atherosclerosis
URI: http://scholarbank.nus.edu.sg/handle/10635/24860
ISSN: 00219150
DOI: 10.1016/j.atherosclerosis.2005.04.019
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

7
checked on Dec 6, 2017

WEB OF SCIENCETM
Citations

6
checked on Nov 21, 2017

Page view(s)

178
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.