Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.aca.2005.09.008
Title: Determination of critical micelle concentrations and aggregation numbers by fluorescence correlation spectroscopy: Aggregation of a lipopolysaccharide
Authors: Yu, L.
Tan, M.
Wohland, T. 
Ho, B. 
Ding, J.L. 
Keywords: Critical micelle concentration
Fluorescence correlation spectroscopy
Lipopolysaccharide
Issue Date: 2006
Source: Yu, L., Tan, M., Wohland, T., Ho, B., Ding, J.L. (2006). Determination of critical micelle concentrations and aggregation numbers by fluorescence correlation spectroscopy: Aggregation of a lipopolysaccharide. Analytica Chimica Acta 556 (1) : 216-225. ScholarBank@NUS Repository. https://doi.org/10.1016/j.aca.2005.09.008
Abstract: Fluorescence correlation spectroscopy (FCS) is often used to determine the mass or radius of a particle by using the dependence of the diffusion coefficient on the mass and shape. In this article we discuss how the particle size of aggregates can be measured by using the concentration dependence of the amplitude of the autocorrelation function (ACF) instead of the temporal decay. We titrate a solution of aggregates or micelles with a fluorescent label that possesses a high affinity for these structures and measure the changes in the amplitude of the ACF. We develop the theory describing the change of the ACF amplitude with increasing concentrations of labels and use it to fit experimental data. It is shown how this method can determine the aggregation number and critical micelle concentration of a standard detergent nonaethylene glycol monododecyl ether (C12E9) and a lipopolysaccharide (LPS: Escherichia coli 0111:B4). © 2005 Elsevier B.V. All rights reserved.
Source Title: Analytica Chimica Acta
URI: http://scholarbank.nus.edu.sg/handle/10635/24854
ISSN: 00032670
DOI: 10.1016/j.aca.2005.09.008
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