Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jmb.2008.01.045
Title: The Macromolecular Assembly of Pathogen-Recognition Receptors is Impelled by Serine Proteases, via Their Complement Control Protein Modules
Authors: Le, Saux A. 
Koh, J.J.Y.
Low, D.H.P.
Leong, G.E.-L.
Ding, J.L. 
Ng, P.M.L.
Ho, B. 
Keywords: innate immunity
pathogen-recognition receptor assembly
protein-protein interactions
serine proteases
yeast two-hybrid method
Issue Date: 2008
Source: Le, Saux A., Koh, J.J.Y., Low, D.H.P., Leong, G.E.-L., Ding, J.L., Ng, P.M.L., Ho, B. (2008). The Macromolecular Assembly of Pathogen-Recognition Receptors is Impelled by Serine Proteases, via Their Complement Control Protein Modules. Journal of Molecular Biology 377 (3) : 902-913. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jmb.2008.01.045
Abstract: Although the innate immune response is triggered by the formation of a stable assembly of pathogen-recognition receptors (PRRs) onto the pathogens, the driving force that enables this PRR-PRR interaction is unknown. Here, we show that serine proteases, which are activated during infection, participate in associating with the PRRs. Inhibition of serine proteases gravely impairs the PRR assembly. Using yeast two-hybrid and pull-down methods, we found that two serine proteases in the horseshoe crab Carcinoscorpius rotundicauda are able to bind to the following three core members of PRRs: galactose-binding protein, Carcinolectin-5 and C-reactive protein. These two serine proteases are (1) Factor C, which activates the coagulation pathway, and (2) C2/Bf, a protein from the complement pathway. By systematic molecular dissection, we show that these serine proteases interact with the core "pathogen-recognition complex" via their complement control protein modules. © 2008 Elsevier Ltd. All rights reserved.
Source Title: Journal of Molecular Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/24847
ISSN: 00222836
DOI: 10.1016/j.jmb.2008.01.045
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